The principle of the vacuum blood collection tube is to pre-extract different levels of vacuum in the blood collection tube with a capped head, and use its negative pressure to automatically collect a quantified amount of venous blood. One end of the blood collection needle is inserted into the human vein, and the other end is inserted into the rubber plug of the vacuum tube. The vacuum blood collection tube is a disposable, negative pressure, vacuum glass or plastic tube that can achieve quantified blood collection, usually used in conjunction with a venous blood collection needle.

Selection and Injection Order of Vacuum Blood Collection Tube

According to the tested item, select the corresponding tube. The order of disposable injection syringe is culture bottle, normal tube, tube containing solid anti-coagulant, and tube containing liquid anti-coagulant. The purpose of following this order is to minimize analysis errors caused by sample collection. Blood distribution order: (1) Using glass tubes: blood culture tubes, serum tubes without anti-coagulant, sodium citrate anti-coagulant tubes, and other anti-coagulant tubes. (2) Using plastic tubes: yellow blood culture tubes, blue sodium citrate anti-coagulant tubes, serum tubes with or without coagulation activator or gel separation, green heparin tubes with or without gel, purple EDTA anti-coagulant tubes, and grey tubes with glucose decomposition inhibitor.

Blood Collection Site and Posture using Vacuum Blood Collection Tube

For infants, blood can be collected from the thumb or side edges of the heel, or from the jugular or anterior fontanelle veins if necessary. For adults, choose veins without congestion or swelling on the elbow, back of hand, wrist, etc., and individual patients may have veins on the back of the elbow. Outpatients are usually seated, while inpatients are usually lying down. Instruct patients to relax during blood collection, keep the environment warm, prevent venous constriction, avoid excessive binding time, and prohibit arm slapping, which can cause local blood concentration or activate the clotting system. Try to choose large, easily fixable vessels for puncture, and ensure that the needle penetrates the vein at the first attempt. The angle of needle insertion is generally 20-30°. If blood return is not seen after puncture, advance the needle slightly forward and then attach the vacuum tube. In individual patients with low blood pressure, blood may not return after puncture, but blood will flow out smoothly once the negative pressure tube is attached.

Strictly Check the Validity Period of the Blood Collection Tube

The tube provided by blood collection tubes manufacturer must be used within its validity period and must not be used if there are foreign objects or sediment in the tube.

Correctly Paste the Barcode on the Vacuum Blood Collection Tube

Print the barcode according to the doctor's order, check it before pasting it on the front of the tube, and the barcode must not cover the calibration of the blood collection tube.

Timely Submit the Vacuum Blood Collection Tube for Inspection

The blood sample collected in the vacuum blood collection tube should be submitted for inspection within 2 hours to minimize the impact of external factors. When submitting the sample, avoid strong light, wind, rain, freezing, high temperature, shaking, and hemolysis.

Storage Temperature of Vacuum Blood Collection Tube

The suitable storage environment temperature for the blood collection tube is 4-25°C. If the storage temperature is at or below 0°C, it may cause the blood collection tube to rupture.